Figure 5. CERKL subcellular localization in 661W cells. Cells were stained with the RA anti-CERKL antibody (red) and with TO-PRO-3 for nuclear staining (blue). A-C: CERKL can be located in both the nucleus and the cytoplasm, where it is concentrated in the perinuclear region. Scale bar, 20 µm. D-F: CERKL is absent from nucleoli (arrows), which are stained by the nucleolar marker Fibrillarin (green). Scale bar, 20 µm.