Figure 1. Specificity of the anti-advanced lipoxidation end product antibodies. Representative dot blots showing the specificity of the anti-advanced lipoxidation end product (ALE) monoclonal antibodies to aldehyde-modified proteins. Anti-Nε-(3-formyl-3,4-dehydropiperidino)lysine (FDP-lysine), anti-malondialdehyde (MDA)-lysine, anti-4-hydroxy-2-nonenal (HNE)–histidine, and anti-4-hydroxyhexenal (HHE)-histidine monoclonal antibodies reacted specifically with FDP-lysine human serum albumin (HSA), MDA BSA (BSA), 4-HNE-HSA, and 4-HHE-HSA, respectively.