Table 1 of Luna, Mol Vis 2010; 16:2511-2523. Table 1 of Luna, Mol Vis 2010; 16:2511-2523.
Table 1. Antibodies used for this study along with their source and concentrations.
| Antibody | Species | Form | Dilution | Manufacturer |
|---|---|---|---|---|
| anti-vimentin | chicken polyclonal | purified immunoglobulin | 1:2000 | Millipore, Temecula CA |
| anti-GFAP | rabbit polyclonal | purified immunoglobulin | 1:400 | DAKO, Carpenteria, CA |
| anti-nestin | mouse monoclonal | purified immunoglobulin | 1:50 | Millipore, Temecula CA |
| anti-synemin | rabbit polyclonal | affinity purified immunoglobulin | 1:20 | [38] |
| anti-BrdU | rat monoclonal | purified immunoglobulin | 1:250 | Accurate cheminal, Westbury, NY |
| isolectin B4 | lectin | biotinylated | 1:50 | Vector Labs, Burlingame, CA |
| Secondary antibodies | ||||
| Donkey anti-rabbit | Cy5 | purified antisera | 1:200 | Jackson Immuno Research, West Grove, PA |
| Donkey anti-mouse | Cy3 | purified antisera | 1:200 | Jackson Immuno Research, West Grove, PA |
| Donkey anti-chicken | Cy2 | purified antisera | 1:200 | Jackson Immuno Research, West Grove, PA |
| Donkey anti-rat | Cy2 | purified antisera | 1:200 | Jackson Immuno Research, West Grove, PA |
| Streptavidin | Cy5 | purified antisera | 1:200 | Jackson Immuno Research, West Grove, PA |