Figure 5 of Luna, Mol Vis 2010; 16:2511-2523.

Figure 5. Immunoblot data for nestin and synemin. A: Very faint bands of nestin were visible at 1 day and this increased at 7 days; no band was observed in control retinas (250 kDa). Only one band at the appropriate molecular weight for nestin was observed, confirming antibody specificity. Lightly labeled synemin bands appeared in control tissue with a steady increase between 1 and 3 days of injury and a strong increase by 7 days (arrows). B: Two primary bands (arrows) were detected for synemin, corresponding to the presence and specificity of the antibody for both the H and M isoforms (225 kDa, 175 kDa). The internal control glyceraldehyde phosphate dehydrogenase (35 kDa) was used to ensure equal protein loading.