Figure 2. HSV-1 can successfully grow and replicate in HCE cells. Confluent monolayers of HCE, Vero, and naturally-resistant CHO-K1 cells were infected with HSV-1(KOS)804 at an MOI of 0.01 for 90 min at 37 °C. Innoculums were harvested, fixed, and stained at 0, 12, 18, 24, and 36 h. Data represents the mean of three samples (±SD). A typical plaque formed by Vero and HCE cells stained with crystal violet at 18 h of infection with HSV-1(KOS)804 virus is shown in (B) and (C) accordingly. Plaques were not formed in the HSV-1 resistant CHO-K1 and the lack of plaques are shown in D. Increasing plaque size over time demonstrating progressive infection was confirmed in E.