Figure 1. Monitoring the DTT-induced amorphous aggregation of α-LA by light scattering at 340 nm (solid symbols) and dynamic light scattering
(open symbols). In both experiments α-LA (2 mg/ml) was incubated at 37 °C in 50 mM phosphate buffer, 100 mM NaCl, 2.5 mM EDTA
at pH 7.2 with 20 mM DTT, in the absence (squares) or presence (triangles) of αB-crystallin (1:1 w:w ratio of α-LA: αB-crystallin).
The change in light scattering at 340 nm is shown on the left y-axis and the Z-average hydrodynamic radius of particles (Z-average
radius, Å) measured by DLS over time is shown on the right y-axis.