Figure 4. ELOVL4ΔC engages in the elongase complex. HEK 293T cells were transfected with a pCE-puro HA-1 (vector), pCE-puro HA-KAR, or pCE-puro HA-TER plasmid, and with the pCE-puro His₆-Myc-ELOVL4 or pCE-puro His₆-Myc-ELOVL4ΔC plasmid. Total cell lysates were prepared from the transfected cells and solubilized with 1% Triton X-100. Following immunoprecipitation with the anti-HA antibody, total lysates (1×) and bound proteins (IB: HA, 2×; IB: Myc, 5×) were subjected to immunoblotting with anti-HA or anti-Myc antibodies. IP, immunoprecipitation; IB, immunoblotting; -, vector; K, KAR; T, TER; 4, wild-type ELOVL4; Δ, ELOVL4ΔC.