Figure 3. Macrophage migration inhibitory factor and vascular endothelial growth factor immunohistochemistry after chemical burn of ocular surface. Thirty days after induction of chemical burns on the ocular surface, the murine corneas were stained with antibodies against MIF (A) and VEGF (B). The inflammation almost completely subsided, and irregularly distributed corneal stromal fibers were noted, with a few microvessels (arrow). Both cytokines were readily observable in the corneal stroma and epithelium. Their expression revealed high spatial similarity as demonstrated in the inserted magnified images. In the epithelial cell layer, both MIF and VEGF were heavily expressed in basal cells as compared to cells in the superficial layers. Both VEGF and MIF were positively stained in cells around microvascular structures (asterisk). The inserted magnified image represents the areas indicated by the arrow and asterisk.