Figure 2. Macrophage migration inhibitory factor immunohistochemistry in normal and chemical burn applied to the mouse eye. In normal control eyes, MIF was expressed in the corneal epithelium, corneal endothelium, lens epithelium, and ciliary body epithelium (A, B). However, it was difficult to find any significant MIF staining in the corneal stroma. C: Negative control; secondary antibody staining without primary antibody application. D: MIF expression was detectable in corneal stroma at day 2 after chemical burn induction. However, the infiltrating inflammatory cells were largely negative for MIF expression (inserted figure in panel D is the magnification of area indicated by the clear arrow). E, F: MIF staining on days 7 and 30 after chemical burn. MIF expression in the corneal stroma was increased but cellular infiltration was reduced compared to day 2.