Molecular Vision: Richardson, Mol Vis 2010; 16:2376-2383. Table 3

Table 3. Proteins with altered levels identified with LC-MS/MS in the albumin-bound fraction of AH of patients with FECD.

Protein ID	Protein common name	Protein coverage (%)	# of unique sequences	CV (%) Fuchs	CV (%) normal	Fold change in FED patients	p value
IPI00940791	20 kDa protein	29.2	4	37.0	118.9	−9.7	0.32
IPI00022429	Alpha-1-acid glycoprotein 1	32.3	4	46.1	66.9	−3.7	0.20
IPI00020091	Alpha-1-acid glycoprotein 2	17.9	2	48.2	95.3	−3.2	0.34
IPI00847635	Alpha-1-antichymotrypsin	25.8	8	11.2	64.0	−2.5	0.24
IPI00553177	Alpha-1-antitrypsin	54.3	24	25.7	84.0	1.3	0.60
IPI00166729	alpha-2-glycoprotein 1, zinc precursor	13.1	3	37.9	55.1	−1.8	0.25
IPI00032220	Angiotensinogen	5.0	1	39.1	78.1	−9.4	0.19
IPI00032179	Antithrombin-III	5.4	1	58.0	37.8	−2.0	0.14
IPI00021841	Apolipoprotein A-I	66.3	21	46.2	102.8	−1.5	0.62
IPI00298828	Beta-2-glycoprotein 1	8.4	2	19.1	22.7	−1.1	0.68
IPI00017601	Ceruloplasmin	4.7	2	26.7	138.9	−6.8	0.40
IPI00291262	Clusterin	4.9	2	24.1	17.7	3.8	0.03
IPI00032293	Cystatin-C	18.5	2	16.6	106.1	−3.7	0.36
IPI00168728	FLJ00385 protein (Fragment)	13.4	4	33.8	8.5	1.6	0.19
IPI00026199	Glutathione peroxidase 3	6.2	1	39.9	82.3	1.0	0.98
IPI00796636	Hemoglobin (Fragment)1	2.4	1	13.8	37.1	5.8	0.001
IPI00410714	Hemoglobin subunit alpha	28.2	3	36.5	68.6	1.5	0.35
IPI00654755	Hemoglobin subunit beta	76.9	9	24.7	115.4	1.2	0.74
IPI00022488	Hemopexin	21.2	6	33.3	82.9	−2.9	0.30
IPI00785084	IGH@ protein	29.5	9	24.4	27.5	2.0	0.04
IPI00784865	IGK@ protein	22.5	3	13.6	24.2	2.9	0.002
IPI00829877	IGL@ protein	23.3	3	10.4	22.4	3.1	0.001
IPI00009650	Lipocalin-1	9.1	1	54.9	156.3	−4.3	0.48
IPI00006114	Pigment epithelium-derived factor	22.3	7	37.8	55.1	−1.1	0.75
IPI00013179	Prostaglandin-H2 D-isomerase	32.6	3	63.9	86.5	−1.6	0.52
IPI00878517	Putative uncharacterized protein ALB	4.9	1	23.0	49.4	3.7	0.01
IPI00399007	Putative uncharacterized protein DKFZp686I04196 (Fragment)	14.6	4	24.7	29.5	1.8	0.06
IPI00930442	Putative uncharacterized protein DKFZp686M24218	9.2	3	25.2	29.4	1.8	0.06
IPI00830047	Putative uncharacterized protein ENSP00000374858 (Fragment)	28.3	2	35.2	30.8	2.6	0.04
IPI00022463	Serotransferrin	29.9	15	22.0	80.6	−1.8	0.43
IPI00745872	Serum albumin	69.0	46	32.7	21.1	2.9	0.07
IPI00018381	Tolloid-like protein 1	2.9	1	51.0	37.9	2.1	0.18
IPI00022432	Transthyretin	73.5	13	38.2	135.1	−15.6	0.35
IPI00555812	Vitamin D-binding protein	4.6	1	72.0	16.2	−1.6	0.24

The percent protein coverage (percent of total protein sequence accounted for by peptides identified), the number of unique peptide sequences, the coefficient of variation for each group, the fold change where a positive number indicates an upregulation and a negative number indicates a down regulation in control patients compared to FECD patients, and the p-value are also reported. ANOVA revealed significant differences in females compared to males in both groups indicating potentially important ramifications for increased FECD incidence in females (p≤0.01). Albumin bound–pooled–triplicate injections; n=23.