Figure 1. Endothelial cell loss on rejected corneal allografts. A: Kaplan–Meier survival analysis: 100% rejection rate of allografts (group R), no rejections for syngeneic controls with endothelium (control group) or following mechanical endothelial removal before syngeneic transplantation (group S). B, C: Group R: Endothelium at the time of rejection (day 15 after surgery). B: Staining with Alicarin red S. C: Same cut, DAPI staining of cell nuclei. Superior; Intact host endothelium; Inferior: Graft with endothelium destroyed following immune reaction. Arrow: Suture in the underlying stroma, indicating the graft border. D, E: Control group: Endothelium of untreated syngeneic controls at the corresponding time point. Superior: Intact host endothelium; Inferior: Intact graft endothelium. Arrow: Suture in the underlying stroma, indicating the graft border.