Figure 2. Intrastromal injection of NP2-targeting
inhibits suture-induced corneal lymphangiogenesis but not
hemangiogenesis. A-C: Representative images showing that
corneal suture injury induced a robust neovascular response seven days
after injury. D-L: Representative segments of corneal
whole-mounts (blood vessels in green: CD31/FITC; lymphatic vessels in
red: LYVE-1/Cy3) in eyes treated with NP2-amiR (F, I, L,
mice) or neg-amiR (E, H, K, n=7 mice)
compared with the saline control eyes (D, G, J,
n=8 mice). Arrows: limbus. Original magnification, 100×. M:
Quantification of immunohistochemical staining in the area of
CD31-possitive blood vessels and LYVE-1-possitive lymphatic vessels.
Bars, SEM. The asterisk indicates a p<0.05 versus saline control.
The vasculized area of control group was defined as 100%.