Figure 4. Co-localization of hBest2 with the α1 subunit of Na⁺/K⁺-ATPase at the basal plasma membrane of the RPE. A: Cryosections of human anterior segments were reacted with GA3512 to identify hBest2. B: The localization of Na⁺/K⁺-ATPase was determined using a monoclonal antibody against the α1 subunit. C: Nuclei were stained with DAPI. D: A merge of the images shows that hBest2 co-localizes with Na⁺/K⁺-ATPase at the basal surface of the NPE. note that the α1 subunit of Na⁺/K⁺-ATPase is also found in the plasma membrane of PE cells, where hBest2 is not expressed.