Figure 3. Localization of hBest2 in transfected HEK-293 cells and human ciliary body. A: HEK-293 cells were transfected with pAdlox-hBest2 and hBest2 localized using immunofluorescence staining with GA3512 (Red in A, C, E). B: No hBest2 staining was observed in untransfected HEK-293 cells. C: Although orientation varied, staining of human ciliary body identified the basolateral plasma membrane of NPE cells. Cells in which basolateral membrane staining is particularly evident are labeled by white arrows. E: Staining was absent when the GA3512 was omitted. D and F: are DIC micrographs of the same fields shown in C and E respectively.