Figure 2. Immunoprecipitation of hBest2 from human donor tissues. A: Lysates of human ciliary bodies (hCBs), human RPE (hRPE) cells, HEK-293 cells (HEK control), or HEK-293 cells transfected
with padlox-hBest2 (HEK w/hBest2) were immunoprecipitated with GA3512 coupled to protein A beads or control beads and blotted
back with GA3512. hBest2 was identified in hCB and HEK w/hBest2 when immunoprecipitated with GA3512 beads, but not control
beads. B: Extraneous bands in A were also present in blots when primary antibody was omitted, and likely represent IgG that has leached
from the GA3512-coupled beads.