Figure 2. REST (NRSF) regulated neuronal
genes are activated in the lens. A: Immunoblot detection of
Synapsin 1 in lens and brain (a: Millipore; b: BD Biosciences). B:
of Synapsin 1 transcripts from lens and brain. C:
Representative sequence identifying exon junction in cDNA products. D:
detection of neuronal βIII-tubulin in lens, and brain (a:
rabbit antibody, Sigma; b: chicken antibody, Aves). E:
Amplification of neuronal βIII-tubulin transcripts from lens and brain.
F: Region from complete amplified product sequence identifying
in frame exon junction in amplified transcripts. G:
Amplification of Synaptotagmin 1 and Synaptophysin 1 transcripts from
lens and brain. H: Region from amplified product sequence
identifying in frame exon junctions in amplified transcripts. Asterisks
indicate splice junctions.