Figure 2. RT–PCR products obtained from minigene constructs transient transfection. The mutated (pTARGET-mut) and wild-type (pTARGET-wt) minigene constructs were independently transfected into HeLa cells and human lens epithelial cells (LECs). Forty-eight hours later, total RNA was extracted from transfected cells and analyzed by RT–PCR. The RT–PCR product obtained from pTARGET-wt and pTARGET-mut shows a 687 bp band and a 327 bp band, respectively. The pTARGET-col used as a control shows a negative band.