Figure 4. Signature truncation of βA3-crystallin upon incubation with CHAPS. Upon incubation of βA3-crystallin (50 μg) with CHAPS for 24 h at 37 °C in the presence of BAPNA, an increase in the protease activity was accompanied with autodegradation of the crystallin. Lane 1: 0 h incubation and lane 2: 24 h incubation of the crystallin alone without CHAPS; lanes 3 [0 h] and 4 [24 h]) incubation with CHAPS; lanes 5 (0 h) and 6 (24 h) incubation with Triton X-100; lane 7: incubation with CHAPS plus BAPNA, and lane 8: incubation with Triton X-100 and BAPNA. Note the autodegradation of the crystallin to three truncated species (identified as I, II, and III) in the presence of CHAPS but not in the presence of Triton X-100.