Figure 3. Assay of protease activity in
the βA3-crystallin following treatment with CHAPS or Triton X-100.
Following treatment of βA3-crystallin preparation (50 μg) with either
CHAPS or Triton X-100 and incubation with BAPNA as a substrate, only
the CHAPS-treated crystallin exhibited protease activity. The protease
activity increased during incubation for 24 h at 37 °C as
indicated by the release of p-nitroaniline (yellow) from BAPNA, which
was monitored at 405 nm.