Figure 7. Immunofluorescence staining of Na⁺/K⁺-ATPase α1. A, C: The corneal epithelium has a bottom-up decreasing gradient of expression of the Na⁺/K⁺-ATPase pumps (in red) from the basal layer toward the wing cells in both native corneas (A) and reconstructed corneas (C). B, D: Immunofluorescence staining of Na⁺/K⁺-ATPase α1 of the corneal endothelium in both native (B) and reconstructed corneas (D). Nuclei were counterstained with Hoechst (in blue). Bar, 10 µm.