Table 1 of Desronvil, Mol Vis 2010; 16:2185-2191. Table 1 of Desronvil, Mol Vis 2010; 16:2185-2191.
Table 1. Characteristics of primers used for amplification and sequencing.
| Primer name | Sequence | Melting temp | Product size |
|---|---|---|---|
| Primers for coding exons of COL8A1 (NM_001850) | |||
| Exon 4 Forward | AAGTCACTTGGCCTTGCAG | 59.02 | 456 |
| Exon 4 Reverse | CCCCTCTGATCCCATAATTTAG | 58.84 | |
| Exon 5_1 Forward | ACTTCATTGATGTGAGAGACAATC | 57.34 | 630 |
| Exon 5_1 Reverse | TGGAGCCCCTGGCTTTC | 62.33 | |
| Exon 5_2 Forward | AGGTGCGCCAGGTGTAAAG | 61.24 | 594 |
| Exon 5_2 Reverse | ACTTCACCAAGGAAACCTGG | 59.04 | |
| Exon 5_3 Forward | CAAAGGAGAAGGTGGGATTG | 59.52 | 584 |
| Exon 5_3 Reverse | TGCCTTTCTTAGCCCCGTAG | 61.59 | |
| Exon 5_4 Forward | GAGTGGCAGGACTTCATGG | 59.20 | 777 |
| Exon 5_4 Reverse | TGTACACAATGGTCCAAATTTTC | 58.78 | |
| Primers for coding exons of COL8A2 (NM_005202) | |||
| Exon 1 Forward | CAGGGCTGGCTTGATGAC | 60.36 | 312 |
| Exon 1 Reverse | AGGGAGGCAGGGGATTTG | 62.33 | |
| Exon 2_1 Forward | GGAATGGGTAGATGGGGTC | 59.01 | 599 |
| Exon 2_1 Reverse | AACCAGGTTTGCCTAAGCC | 59.18 | |
| Exon 2_2 Forward | GATAATGGAGTGGGCCAGC | 60.44 | 580 |
| Exon 2_2 Reverse | CACTAGGCCCCTGGTCAC | 59.05 | |
| Exon 2_3 Forward | GCTTCCTGGCAGACGTG | 59.00 | 597 |
| Exon 2_3 Reverse | AGCCCAAACTGTGGCTTG | 59.83 | |
| Exon 2_4 Forward | CTCCCCTGGAATCACGG | 59.98 | 636 |
| Exon 2_4 Reverse | TTGAAAAGGTCGCTCTACCAC | 59.37 | |