Table 1 of Zhou, Mol Vis 2010; 16:2163-2174. Table 1 of Zhou, Mol Vis 2010; 16:2163-2174.
Table 1. Sequence of primer pairs and length of the amplified sequences based on the NCBI database
Gene |
Forward primer (5′-3′) |
Reverse primer (5′-3′) |
Annealing temperature (°C) |
Cycles |
Length of product (bp) |
|---|---|---|---|---|---|
| αA-crystallin | AGCCCTTGCCAGCCATCT | GCTTGTGCCACCTGCTCTTTA | 60 | 33 | 220 |
| αB-crystallin | AGTTCTTCGGAGAGCACCTGTT | TCCTTGGTCCATTCACAGTGAG | 64 | 34 | 321 |
| βA3/A1-crystallin | GCCTGGAGTGGAAGCAAT | CTGGATACGGCGAATAGA | 59 | 35 | 344 |
| βA4-crystallin | TGCTGAGTGGAGCGTGGGTAGG | GTGGACGTGGAAGGAGCCCACT | 65 | 35 | 300 |
| βB2-crystallin | CCAGAACCTTAACCCCAAGATC | GCTGTCCACTTTGATGGGCCTC | 65 | 35 | 277 |
| GAPDH | CGGAGTCAACGGATTTGGTCGTAT | AGCCTTCTCCATGGTGGTGAAGAC | 65 | 30 | 304 |