Figure 7. Level of IL-12p35 and IL-12p40 transcripts in macrophages infected with HSV-IL-4. Subconfluent monolayers of macrophages were infected with 10 PFU/cell of HSV-IL-4 or parental virus. Total RNA was isolated 12 and 24 h post infection and TaqMan RT–PCR was performed using IL-12p35- and IL-12p40-specific primers as described in the Methods. IL-12p35 and IL-12p40 mRNA levels were normalized in comparison to each transcript in mock-infected cells. GAPDH was used as internal control. Each point represents the mean±SEM (n=8).