Figure 6. Level of IL-4 and HSV-1 gB transcripts in RS cells infected with different recombinant viruses. Subconfluent monolayers of RS cells were infected with 10 PFU/cell of HSV-IL-4, vEye2, vEye3, vTG2, or vTG3. Total RNA was isolated 24 and 48hr post infection and TaqMan RT–PCR was performed using IL-4- and gB-specific primers as described in the Methods. In each experiment, an estimated relative copy number of IL-4 and gB were calculated using standard curves generated from pVR1055-IL-4 and pVR1055-gB, respectively. Briefly, DNA template was serially diluted 10-fold such that 5 μl contained from 10³ to 10¹¹ copies of IL-4 or gB, then subjected to TaqMan PCR with the same set of primers. By comparing the normalized threshold cycle of each sample to the threshold cycle of the standard, the copy number for each reaction was determined. GAPDH was used as internal control. Each point represents the mean±SEM (n=4). Panel A indicated gB and panel B indicates IL-4.