Figure 5. Southern analyses of isolated viruses. Subconfluent RS cell monolayers were infected with 10 PFU/cell of KOS, HSV-IL-4, vEye2, vTG2, vEye3, and vTG3 viruses for 16 h. Viral DNAs were isolated, 5 μg of DNA/each virus was digested with BamHI, and hybridized to ³²P-labeled murine IL-4. pLAT-IL-4 containing the full-length IL-4 was used as positive control. Lanes: KOS, HSV-IL-4, vEye2, vTG2, vEye3, vTG3, and pLAT-IL-4.