Figure 3 of Liu, Mol Vis 2010; 16:184-193.


Figure 3. The mTOR/translational pathway was involved in IFNγ-induced VEGF secretion from RPE cells. A: ARPE-19 cells were cultured with or without IFNγ in the presence or absence of rapamycin for 15, 30, and 60 min. Cells were collected and processed for western blot analysis using anti-p-p70 S6 kinase, anti-p-S6 ribosomal protein, anti-p-4E-BP1, p-akt, and GAPDH antibodies. B: ARPE-19 cells were cultured with or without IFNγ/rapamycin for 24 h. Cells were collected for RNA purification. Real-time PCR assay was performed and the results were expressed as the n-fold expression of hVEGF normalized on that of GAPDH or 18S rRNA. C: ARPE-19 cells were cultured with or without IFNγ/rapamycin for 48 h. Cell supernatants were collected and used for ELISA analysis. The values are expressed as the average+SEM of triplicates of each treatment. The results were representative data from three separate experiments. The double asterisk indicates statistical significance (p<0.01) compared to the IFNγ group. D: ARPE-19 cells were cultured with or without IFNγ in the presence or absence of rapamycin for 15, 30, and 60 min. Cells were collected and processed for western blot analysis using anti-p-Stat1, Stat1, and GAPDH antibodies. The results were representative data from two separate experiments.