Figure 9. Effect of recombinant pBTLA on the levels of gD mRNA expression in cornea. Real-time quantitative PCR analysis of gD mRNA levels results are shown for corneas from the different mouse groups (n=6 in each group) at 72 h after HSV-1 infection. RNA was extracted from corneas of mice from the different groups and the levels of gD expression were evaluated through real-time quantitative PCR. A: The melt curves of gD and GAPDH after amplification in corneal samples. B: The fold changes of gD mRNA expression in corneas of different groups at 72 h postinfection. The gD mRNA levels in the corneas of pBTLA treated mice did not differ from those of the pcDNA or PBS control mice (p>0.05).