Figure 5 of Xia, Mol Vis 2010; 16:2071-2083.


Figure 5. Decreased inflammation in the corneas of mice treated with pBTLA. A: pBTLA treated, pcDNA3.1 treated and PBS control mice were infected with 1×105 PFU of HSV-1 KOS. On day 14 postinfection, mice were euthanized, and eyes were processed for cryosectioning. H&E staining was conducted on 5-µm sections. The corneas in the pcDNA3.1 treated and PBS control groups show severe swelling, heavy infiltration with inflammatory cells, and numerous neovascular structures in the stroma. The cornea in the pBTLA treated group exhibits only mild stromal edema, little inflammatory cell infiltration, and fewer neovascular structures. Original magnification, 400×. B: Six corneal samples from each group of animals were digested with liberase on day 14 post infection. The bars represent the total number of viable cells present per cornea of different groups of mice. *p<0.01 compared with control groups. C: The cells isolated from infected corneas were stained for CD4 marker and the bars represent the total number of CD4+ T cells present per cornea from different groups of mice. Decreased numbers of CD4+ T cells in the cornea of pBTLA treated mice. *p<0.01 compared with control groups. D: The percentage of CD4+ T cells in total cells of per cornea from different groups of mice. *p<0.01 compared with control groups. E: Representative plot shows CD4+ T cells expression on cornea of different group mice.