Table 2 of Ma, Mol Vis 2010; 16:2043-2054.

Table 2. Primers designed for mutation screening.



Exon

Primer Sequence (F,R)		 Melting
Temperature
(°C)		 Product
Size
(bp)
CDH6
1 F:CAGACGGAGTCATACAAGTTCTGAG 58 824
  R: GCCCTTTGGTAATTGACCAGC 59  
2 F:GCACATGCCTTCCATTTAGC 57 770
  R: GGTTGTGGGTGTTTCAACTGG 59  
3 F: CTCCCAAACTCTGTTCCAGTTC 57 775
  R: TCTCTTTCAACCTCCCACTCC 57  
4 F: CCAAAGTTCTCGACTTCCTCAG 57 369
  R: GTGTTTGGTGGATGTATGCAAG 57  
5 F: ATCTATCTCCCCTGTGTGGTTG 57 521
  R: TTCCTGAGTGTATGCCATGTTG 57  
6 F: AAGAAGAACAGGCCACCATTAG 56 583
  R: GGTTTTGCCATGTTGGTCTC 57  
7 F: CATTTTTCAGGGCTGTGTGG 58 592
  R: CATCTTTTCTCAAGTGCAGGC 56  
8 F: GGTGATCTTCAAAGTCATGCAAC 57 538
  R: GAAACATTACTGCAAACCACTCC 57  
9 F: GTCTAAAGGGAATCGGCAATC 56 397
  R: TGGAATCAGCCTCAGTCTTTG 57  
10 F: TACTGATATCTCGTGGGTAGAGGC 58 552
  R: GCAAAGTGGTGAATGTATGTGG 57  
11 F: GCCAGTGGCTCAAACTTTACC 57 573
  R: CAGGCTGTATGCCTTAATGGG 58  
12 F: ATCATGGATGGAGGCAAGTG 57 756
  R: AACGGGTAGAACAGAGAAGCC 58  
CDH10
1 F: CTATCAGCAGAACCTTTCTCTCCG 60 562
  R: CAAACATTTATCTCCTCCCTCTCC 58  
2 F: CACAACAGAAGGCGTGATTCC 59 603
  R: TGCTTCCTCACTGAACTCAATAGC 59  
3 F: TTTACCAAGCAAAGACAGGAGC 57 672
  R: CTCATGGTAGCAAATCAAAGAGG 57  
4 F: TTGCTCCTCCTTCTGGTACTGTG 59 626
  R: TTCATGTTCGGTAAAGCAGTCC 58  
5 F: GTGGTATTGCTAGGAAAGGGTAAC 56 600
  R: GGATCATAGGTCTTCCTGTCTCTG 57  
6 F: AAAAGCCCCGGAAGTTCCTAG 60 611
  R: CAGGTTTCCTGTCTCAATCAACC 59  
7 F: TGTAACTGGGTGGGAGCATATC 58 467
  R: AGTAGAGACAGGGTTTCACCATG 56  
8 F: TCAGTGATATGTGTGGGTTTGC 57 521
  R: CGGCCTGTTAATCTGTTTCATG 58  
9 F: CACTTCATACCCACAAGATGCC 58 577
  R: GCATTCGTCTCTCATCTCTCTAGC 58  
10 F: TAAAGGGTATGATCCAAAAGACAC 56 587
  R: ATCTCCAGCCGTTCTAATCTTATC 56  
11 F: GTAAGCACACACGCACAGATG 56 997
  R: TTTCCAAGCTCCTACACATGC 57  
12-1 F: TGCTAAACCCTTCAGCGTCTC 58 779
  R: AAATTGTGCTGACTGGCAGG 57  
12-2 F: TCCATTGCTGAATCTCTGAGTTC 57 889
  R: CATAGCATATCAAGACTCGCTGG 58  
CDH12
1 F: CAGGTGACAGTTCTCTGATATGC 55 679
  R: ATCCCAATCAAAAACGGAAG 55  
2 F: CAATAGTGATAATCAGGTGTGAGG 58 398
  R: TTGTTGTGTTTATGTCAACTCCTTG 57  
3 F: TGCTGATTAGGATGTGGGC 56 403
  R: TCCAGTCTGGGTGAAAGAGTG 56  
4 F: AGCGTTCTTGCTAATCAGGTC 55 367
  R: GAAATTCAGTGCCATGCAGTC 57  
5 F: GGCAGATATAATGAGCGTTGTG 56 537
  R: CCTTCCTATCAAGCGGTTGTC 57  
6 F: TGGCACATCCTTTTAATGGTG 57 421
  R: TGTTGAAGAGGTCTCATTGTATCTC 55  
7 F: CGAGTCCAGCAGATAAGAGTCATG 59 233
  R: GGCTGGTGATAATGTTGCCTC 58  
8 F: ATATTTCTCATTGTGGCATGGC 58 511
  R: GCTTCCTAAAGACTAAGTGTCTGG 57  
9 F: GCCCAGTTAAAATTCTAGAGCAGC 59 560
  R: CACGGAGTATCAGTACCCCAAC 57  
10 F: TCACCATTTCTGCCACATTC 56 414
  R: CATCATGCAGTTTTGGACAGAG 57  
11 F: TTTCCCCTTGAGCATACTGAC 56 399
  R: GAAAAACATCTCAGCAGGGAC 55  
12 F: TGTAGAAGCAATAACTGACCGG 56 384
  R: TCATCTGTGCGGTATCACCTC 57  
13 F: CCTCTTTGAAACTGATGACCG 56 366
   R: ACAATGCAAGCAACCTGCC 58  
14 F: CTATTGAGCAGATACCAACTTGAAG 55 385
  R: AAAAAAAGGAAGAGAGAGCGAG 55  
15-1 F: CATTCACGAAACTCAGCCAC 55 521
  R: GATAGTCATAGTCCTGGTCGGC 56  
15-2 F: CTGCCCCACCATACGATTC 57 494
  R: ATAGGCCTGAGCTTGTCTCAG 55  
15-3 F: TCTGCCAACAAGAGATACATCC 57 632
  R: ATTTGAGCCCTGAGGCCTC 58  
PDZD2
1-1 F: CTCTTCCTCTCCCAGGTGTGA 58 410
  R: AGGTGCAGCACGGCATTG 60  
1-2 F: AGCCTGAACATGAACACAGGC 59 776
  R: TGGTGCTCCAGTTGAAGATGTG 60  
2 F: AAGCTCAATACCTCAGTCCATCG 58 720
  R: CAGCTTGTATTTCCCGCATG 58  
3 F: CCAGTTGCCACTAGCCACATC 59 615
  R: TGTGCTCAGAGGTGTGTTCAATT 58  
4 F: AACAATCGCATCCCCAACTG 59 587
  R: TGCGCCATTGCACTCCAG 61  
5 F: CCTGGCCTTTGAGGTAACCTT 58 425
  R: TGGTGTCTTTTCCCATCATGGT 60  
6 F: GGAATTGGCCAATGCAAGG 60 671
  R: TTGCTCCAAAAGCTGCAACTC 59  
7 F: GCCTGACACTAATGGCTTCAGC 60 630
  R: CTATGAGACGCCATCGTCTCC 58  
8 F: TTTATTAGAGCCGGGGTTTCAC 58 639
  R: TGCAATGGCTCATGCCTATAATC 60  
9 F: TTAACTCCAGGCCTGGTTAGGG 60 592
  R: CCAGCCCTAAGGTAAGAATGGAC 59  
10 and 11 F: GTGACATTCCTGGGAGCTAAGCTA 60 816
  R: AGAGCTCCATGCTACTGGTAACTTC 58  
12 F: GCCATGGGCTCATTCTATTAACAG 60 529
  R: GGGAAGCCAAGATCTAGAGTTCTC 58  
13 F: GCATAATGACCTTTGCCCACTT 59 575
  R: CTTACCTCCCACCAGACAAGTTTC 59  
14 F: GGAGGATGGTCATAATCCTTGG 58 691
  R: CTATCTCAAGCCTTCTCTGCCTG 58  
15 F: CTGGGATCATCATGCAGGTAGTAG 59 584
  R: TAAAGCCCCAAGTCCTGACTCAC 60  
16 F: GTTGTCAATTTAGCCGTTCTGAG 57 579
  R: AGTAGAGATGGGGTTTCACCATGT 59  
17-1 F: AGCCATGTTGCCTAGGCCTT 60 583
  R: CCATGCCCTCTGGGATACTC 58  
17-2 F: GACAGAGAAGGGGACTGCATT 57 725
  R: CCAGCCATCATCCTTTCCC 59  
18 F: TGGATCTGCTGGCTCCTAGTC 58 430
  R: TCTCAAACTCCTGACCTCAAGTG 57  
19-1 F: GCTGGTCTCGAACTCCTGACC 60 657
  R: AGGTGCATGGATTCCTGTCATT 59  
19-2 F: ACAATACCAGGAGGGTGGCTG 60 820
  R: CTTGTGCAAAGACACACGGG 59  
19-3 F: GACAGCACCTCCCTATCAGGC 60 800
  R: CATGATGGGCCTCCTAGCG 60  
19-4 F: GCATTAATGCAGCTGCCAGTC 59 705
  R: GCGAGACAAATGTCCTGATGC 59  
19-5 F: TGACAGAAACACCACAGCTGC 58 721
  R: GTATTCTGCCTTTCTAATGGCTG 56  
19-6 F: AATCAGGCTCTATCGCCAGGT 59 630
  R: ATCCGCTGCTTCACAGAGAA 57  
19-7 F: TATATAGTGTAAAGCCGCTGCTGG 59 779
  R: TGGGAACTCTGCATTATCTTTGC 59  
20 F: ATTCATAGCAGTTCCCCTTGCC 60 520
  R: CTGAAGCTGGCTAGCAGCAC 58  
21 F: AGAACCTTTAGGGCCTGTGG 57 638
  R: AAGGTGACCCTCTGGATGGTC 59  
22 F: GAGGCTGAGATTGCACCACTG 59 765
  R: CCTTACCAGTCCTAACAAGAGGC 57  
23 F: AGTGCTACTGGGCTCAAGTGC 58 511
  R: GGGATAATGATGACACCCACC 57  
24-1 F: ACAGATTATGTTTGGAGGGGC 57 675
  R: TCACATCTTGTATCCCCATCAGTA 57  
24-2 F: TGTGCAACAGCAATGAAATTAAC 56 732
  R: TGCTCTTGGACTGACCAGTC 55  
24-3 F: TAGAGGGAGCAGAAAGGTCAACA 59 771
  R: TCATGCACACAGGTATGGCAA 60  
24-4 F: GTAAAGGAGCAGAAATGTAGTTACA 56 722
  R: AGGTCTACCCTTGTACTCCAGATAT 55  
24-5 F: TTAATTAATAAACGCACAGCCCTA 56 844
  R: GTACCTCTGATGCATTTAGGTGAC 56  
GLOPH3
1 F: TAATTAACTCCCGCGCCGA 60 776
  R: GGGGAGGATCCAGAAAGCA 59  
2 F: TGGGGTTAACTGAGTATTCCTTGG 59 814
  R: TATTGTCCTGTGACCCTGCCA 60  
3 F: GCTACTGAGTCTAGCCAATTTTCAT 56 637
  R: TACCACCACAGCTTAACCTAGCC 58  
4-1 F: GGTCTGGCTAGGCTTAAGGGG 60 613
  R: GAATGGTTCACCCCGAGCA 60  
4-2 F: AAGAGAGTGCGGCAGCTTCTC 60 734
  R: CCCATCCCAAACTGGCTCT 58  
4-3 F: GGCCTTCAACTCACCAAAGGTA 59 679
  R: TACATGCAACATCTGCTAGGACTG 58  
4-4 F: GGCTTGTGACCAGTACCAATCT 57 578
  R: AAACACAAATGACATGCTTGCTC 58  
ZFR
1 and 2 F: TTAAGGAGCCGCGAAGACG 60 664
  R: TTCGTCGCATCGACAGGAT 59  
3 F: GACCTTTTGTGGTCCGTCATT 57 578
  R: GGTATGTCCCAAACTACCAAGG 56  
4 F: GCCAGGATGGTCTTTATCTGCT 58 664
  R: CAGCTTATTTCAGCAGGAATGG 58  
5 F: GGAGAAATTGCTGGCATAAAAT 56 603
  R: CTAAGCCCAAGACTCATAATGAGC 57  
6 F: AAGGTTCTTCAAGGCAGGGAC 58 667
  R: CCCTGAAAATTCTCATGCCAC 58  
7 F: CGGGCGATACAGAGAACCATAG 59 568
  R: GACAGGTCTCCAGTCTTTCCCTC 59  
8 F: GAGGGAAAGACTGGAGACCTGTC 59 893
  R: AGGGTCTTTGTGTGTGCAGATC 58  
9 F: GATGAGGAGGGTGTTGGGTG 59 675
  R: TGCACACCACAGTGGCAATAC 59  
10 F: GAGGTTGTAGTGAGCTGAGTTCAA 56 486
  R: AACCAAACATCCATCTGAGTCTAC 55  
11 F: TACATGTAGATTGTTTTGGGGC 55 492
  R: TTGTTTGAAACCGAGGCACT 57  
12 F: TGGGAAGAAATTTTAGCTAGGCTG 59 516
  R: AAGCTGAGGCAGGAGAATTGCT 60  
13 F: GGTGTACATGCATGCATGCAT 59 569
  R: TGCAAGCAGCTGCAGAATACAT 60  
14 F: GATGGAAATTTTAATGGCACAAA 57 573
  R: TCCTAACAACTGCCTTCTTATGAT 55  
15 F: AATAATACTGGCATGTACGGCAG 57 406
  R: ATGCCAGCATGTTGCCTTCTA 59  
16 F: GGCTCATGTGACACTGATGCTAC 58 366
  R: GCAATATGCAGATCATCATACCC 57  
17 F: CTGAGCTTCCATTGAACGGTG 59 335
  R: CCCAGGATTTTTCATCAGAAAAG 58  
18 and 19 F: GATTACAGACGTGAGCCACTGTG 58 666
  R: TCTAGGGGCTTGCTTACTACAGA 56  
20-1 F: TAGGTTGCATTTGGAGGGAGG 60 774
  R: CAAACTCTGCAGTCTCACGTTACA 58  
20-2 F: CGAGATGGTATCCTTTACCCC 56 570
  R:CACACCAATAAGGAACTGTCACC 57  
20-3 F: CTTGTGTATAAGTGGAAAGGGCA 57 594
  R: GGCCGTGCTTAGACAACAAAC 58  

Forward and reverse primers designed for CDH6, CDH10, CDH12, PDZD2, GLOPH3, and ZFR for mutation screening.

Ma, Mol Vis 2010; 16:2043-2054. http://www.molvis.org/molvis/v16/a220

©2010 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535