Table 2 of Chen, Mol Vis 2010; 16:2016-2025. Table 2 of Chen, Mol Vis 2010; 16:2016-2025.
Table 2. Primer sequences and PCR conditions for SPARC sequencing.
| Primer sequence | |||||
|---|---|---|---|---|---|
Amplifying target |
Forward primer (5′→3′) |
Reverse primer (5′→3′) |
MgCl2 (mM) |
Ta (°C) |
Size (bp) |
| SPARC-1 (promoter + exon 1) | CCAGTTCCAAATCATCAAGGA | GGGGTTGGTGCAACTATAGAA | 1.5 | 59 | 668 |
| SPARC-2 (exon 2) | AAATGGAACCAACCTCCTCA | CAATGGTCCTCATCCCAGTT | 1.5 | 60 | 388 |
| SPARC-3 (exon 3) | AGCTCCCCTAGCCTGTATCC | CCCTAATTTCTCAGGGCACA | 1.5 | 60 | 225 |
| SPARC-4 (exon 4) | CTTTCCCTAACACCCCTGGT | TCATGTAGGCTGTCCTCGTG | 1.5 | 60 | 367 |
| SPARC-5 (exon 5) | TGTGCTAGTCCAGGTGATGC | TGTATTCCGAAGTGCCCAAT | 1.5 | 60 | 222 |
| SPARC-6 (exon 6) | CAGTGTCCCCATCTCTGAAA | CCCAAGACAGGAGTCTGGAA | 1.5 | 60 | 250 |
| SPARC-7 (exon 7) | AAGAAACTGTGGCCTGGAGA | CTGGTGCTCAGGGGTAAATG | 1.5 | 60 | 396 |
| SPARC-8 (exon 8) | CTGGCTAGTCTCTGCCTGCT | TCACTCTAGGGTCTGGGGTCT | 2.0 | 60 | 279 |
| SPARC-9 (exon 9) | GGGTGTGGAGCTTTTCCAT | CCCCTTGCTTCTTTGTTCAG | 1.5 | 60 | 229 |
| SPARC-10 (exon 10) | TCCACTGACTCCTTGGGAAG | GGCAGAACAACAAACCATCC | 1.5 | 60 | 198 |