Figure 4. H-RN inhibits angiogenesis of chorioallantoic membrane model. A-D: Representative photographs of antiangiogenesis in chick chorioallantoic membrane (CAM) of H-RN, H-FT, and scrambled peptide. Filter paper disks saturated with H-RN (10 µg, 50 µg), H-FT (10 µg, 50 µg), scrambled peptide (10 µg, 50 µg) or PBS were placed on the CAMs for 72 h of incubation. Vessels around the filter disks were photographed: A, Control (PBS); B, 50 µg H-RN per egg; C, 50 µg H-FT per egg; D, 50 µg scrambled peptide per egg. (magnification was ×11.5). E: Quantitative results showed different effects of two peptides on CAM angiogenesis. H-RN inhibited CAM angiogenesis significantly, compared to the PBS group. No such antiangiogenesis activity of H-FT or scrambled peptide was found (** p<0.01, each condition versus control).