Figure 6. Inflammatory changes in recoverin-immunized B6.MRL-Fal^lpr/J (LPR) mice and hybridoma balb/cJ mice. Increase of C1q and C3 deposition and infiltration of CD3+ and CD68+ cells in the retina of recoverin-immunized LPR mice and Balb/c mice injected with recoverin hybridoma cells. No C1q deposition and C3 deposition in only retinal pigment epithelium (RPE) was observed in control LPR mice, while they were enhanced in the RPE, inner nuclear layer (INL), and ganglion cell layer (GCL) of recoverin-immunized LPR mice retina. No C1q or C3 deposition in RPE was observed in control Balb/c mice, while some C1q deposition was observed in the RPE, INL and GCL of the retina of Balb/c mice injected with recoverin hybridoma cells, and a marked increase of C3 deposit on RPE and INL was observed. CD3+ cells were observed between the INL and GCL layers, and the migrating cells between INL and GCL were CD3+ cells. While CD68+ cells were found in inner plexiform layer (IPL) and outer plexiform layer (OPL) in recoverin-immunized LPR mice retina, they were found in RPE and OPL in Balb/c mice injected with recoverin hybridoma cells. Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI; blue). Abbreviations: ONL, outer nuclear layer. Scale equal to 50 µm.