Figure 5. Immunohistogic identification of retinal changes in recoverin-immunized B6.MRL-Fal^lpr/J (LPR) mice and balb/cJ mice injected with recoverin hybridoma cells. Glial fibrillary acidic protein (GFAP) staining detected a marked increase of Muller cell reactive gliosis in recoverin-immunized LPR mice and balb/cJ mice injected with recoverin hybridoma cells, which indicated retinal degeneration. Rhodopsin and S-opsin expression were not altered in these groups, while bipolar cells (protein kinase alpha [PKC-α]) were decreased in both test groups. There is no change in amacrine cells (calretinin) in these two mouse models, however the inner plexiform layer were reduced. Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI; blue). Abbreviations: GCL represents ganglion cell layer; IPL represents inner plexiform layer; INL represents Inner nuclear layer; ONL represents outer nuclear layer; RPE represents retinal pigment epithelium. Scale bar equal to 50 µm.