Figure 2. Accelerated anti-recoverin antibodies in recoverin-immunized B6.MRL-Fas^lpr/J (LPR) mice associated with attenuated electroretinogram (ERG) responses. Although there was an increase of anti-recoverin antibodies in recoverin-immunized C57BL/6J mice, the ERG responses were still normal compared with control C57BL/6J mice. The antibody level was significantly enhanced in recoverin-immunized LPR mice compared with recoverin-immunized C57BL/6J mice, accompanied by attenuated scotopic and photopic ERG responses. A serum titer (1:10,000 dilution) of anti-recoverin antibodies was analyzed by enzyme-linked immunosorbent assay (ELISA; upper) and western blot (middle); ERG responses were also analyzed (bottom). There were significant differences in antibody levels by ELISA in recoverin-immunized LPR mice versus control LPR mice (p<0.001); recoverin-immunized LPR mice versus recoverin-immunized C57BL/6J mice (p<0.001); recoverin-immunized C57BL/6J mice versus control C57BL/6J mice (p<0.05).