Figure 1. Purification of recombinant recoverin and generation of recoverin hybridoma cells. A: Sodium dodecyl sulfate PAGE (SDS–PAGE) analysis of the fractions during the recoverin purification process. 1) crude extract; 2) after centrifugation; 3) after filtration; 4 and 5) apply to column; 6) wash column; 7–11) elution. B: Immunoblot analysis of the eluted fractions of recoverin. C: Immunoblot analysis of the ascites from three balb/cJ mice injected with recoverin hybridoma cells. Molecular size standards are shown on the left.