Table 2 of He, Mol Vis 2010; 16:1913-1919. Table 2 of He, Mol Vis 2010; 16:1913-1919.
Table 2. PCR assay conditions.
| Value | ||
|---|---|---|
| PCR assay conditions | First-step PCR | Second-step PCR |
| QNRT–PCR | ||
| Initial denature | 93.0 °C, 5 min | |
| Amplification | ||
| Denature | 93.0 °C, 30 s | |
| Annealing | 55.0 °C, 45 s | |
| Extension | 72.0 °C, 45 s | |
| Final extension | 72.0 °C, 7 min | |
| Incubation | 93.0 °C, 3 min | |
| Initial denature | 95.0 °C, 10 min | |
| Amplification | ||
| Denature | 93.0 °C, 30 s | |
| Annealing-extension | 55.0 °C, 45 s | |