Table 1 of He, Mol Vis 2010; 16:1913-1919. Table 1 of He, Mol Vis 2010; 16:1913-1919.
Table 1. Sequences of primer and TaqMan probes for PCR assays.
| Object | Type | Sequence |
|---|---|---|
| QNRT–PCR assay | ||
| First-step PCR | outer forward primer | 5′-TGACGGACCTGGATGTGGT-3′ |
| outer reverse primer | 5′-TGTCATTCCCGTAGCAGTAGGA-3′ | |
| Second-step PCR | TaqMan forward primer | 5′-TGCGTCCCACGACATCTG-3′ |
| TaqMan reverse primer | 5′-CAGGTGAATGGGCACATGTG-3 | |
| TaqMan probe-mutation-FAM | 5′-FAM-ATCGATTGGAGAGGCCGGACCG-TAMRA-3′ | |
| Internal control | Human-β-actin forward primer | 5′-GCATGGGTCAGAAGGATTCCT-3′ |
| Human-β-actin reverse primer | 5′-TCGTCCCAGTTGGTGACGAT-3′ | |
| TaqMan probe-mutation-FAM | 5′-FAM-CCTCACCCTGAAGTACCCCATCGAGC-TAMRA-3′ | |