Figure 2. Toll-like receptor 4 deficiency suppresses induction of proinflammatory markers after ischemia/reperfusion. A: Differential expression of proinflammatory molecules in the retinas of wild type (WT) and toll-like receptor (Tlr4) knockout (KO) animals 24 h after ischemia/reperfusion (IR). Gene expression was assessed using real time PCR in sham-operated controls and experimental retinas following IR. For each gene, results are expressed as a percentage of corresponding value in the sham-operated eye±SEM after normalization to β-actin. (*p<0.05, n=6). B: The levels of p65 (RelA) in Tlr4-deficient mice after retinal IR injury were studied using real time PCR and western blot analysis. C: Immunohistochemistry for interleukin 6 (Il6) and chemokine (C-C motif) ligand 2 (Ccl2) protein accumulation in post-ischemic retinas of WT and Tlr4 KO mice are consistent with increased levels of the transcripts at the level of corresponding proteins. Scale bar equal to 100 μm.