Figure 1. Toll-like receptor 4 deficiency results in neuroprotective effects in the ganglion cell layer of retinas after ischemia/reperfusion. A: The percent surviving of ganglion cell layer (GCL) neurons in the ischemia/reperfusion (IR) retinas of wild type (WT) and toll-like receptor 4 (Tlr4) knockout (KO) animals seven days after IR. The percent of Neuronal Nuclei (NeuN)-labeled neurons in regions of central, middle and peripheral retina were compared between sham operated and ischemic eyes of WT and Tlr4 KO animals seven days after IR (**p<0.01, n=6). B: Representative confocal images of NeuN-labeled GCLs (green) in flat-mounted controls and ischemic retinas seven days after reperfusion. Scale bar equal to 100 µm.