Table 1 of Strzalka-Mrozik, Mol Vis 2010; 16:161-166. Table 1 of Strzalka-Mrozik, Mol Vis 2010; 16:161-166.
Table 1. Characteristic of primers used for amplification.
Gene |
Sequence of primers |
Length of amplicon (bp) |
Tm (ºC) |
|---|---|---|---|
| GAPDH | Forward: 5’-GAAGGTGAAGGTCGGAGTC-3’ | 226 | 80 |
| Reverse: 5’-GAAGATGGTGATGGGATTC-3’ | |||
| TGFβ-1 | Forward:5’TGAACCGGCCTTTCCTGCTTCTCATG3’ | 151 | 85 |
| Reverse: 5’GCGGAAGTCAATGTACAGCTGCCGC3’ | |||
| TGFβ-2 | Forward: 5’TACTACGCCAAGGAGGTTTACAAA3’ | 201 | 80 |
| Reverse: 5’TTGTTCAGGCACTCTGGCTTT3’ | |||
| TGFβ-3 | Forward: 5’CTGGATTGTGGTTCCATGCA3’ | 121 | 81 |
| Reverse: 5’TCCCCGAATGCCTCACAT3’ |