Figure 4. Effects of mapracorat and
dexamethasone on hyperosmolarity-induced phosphorylation of p38 and JNK
in T-HCEpiC. Cells were cultured in complete (HCGS containing) medium,
followed by basal medium for 20 h. Cells were pre-treated with
dexamethasone or mapracorat for 2 h. Cells were then treated with 440
mOsm hyperosmotic media + dexamethasone or mapracorat for 30 min.
Phosphorylated p38 (A) and JNK (B) concentrations were
determined by cell-based ELISA. Data are presented as mean±SEM, n=6.
*Versus 440 mOsm media; p<0.05.