Figure 2. Increased levels of deleted mtDNA in aged rat lens. Measurements of levels of the PCR products of non-deleted (A) and deleted (B) mtDNA normalized by total mtDNA were done using the EvaGreen reagent. A: there were no significant change in non-deleted mtDNA at 16 months (p=0.51, n=8) and at 26 months (p=0.93, n=8), compared with the 2 month group. There was not a significantly change at 26 months compared to 16 months group (p=0.56, n=8). However, there was a significant increase in deleted mtDNA at 16 months (p=0.002, n=8) and at 26 months (p<0.001, n=8), compared with the 2 month group in B. There was a significantly increase at 26 months (p<0.01, n=8) compared to 16 months group.