Figure 2. Comparison of primary sphere formation by rabbit CEC from the periphery and center of the cornea. A: The number of spheres obtained from limbal CEC (n=10) was significantly higher than that from central CEC (n=10) after 7 days of culture. The experiment was repeated twice and representative data are shown. The asterisk indicates a p=0.0028 (unpaired t-test). B: The mean sphere size exceeded 250 μm on day 7.