Figure 1. Arrangement of eyes for magnetic resonance imaging scanning. The figure shows 16 paraformaldehyde-fixed chicken eyes embedded in low-melting-point agarose in a 2×2×4 array in readiness for an overnight magnetic resonance imaging (MRI) scan. Note that the eye in the lower-right quadrant of the uppermost layer was positioned in an inverted orientation to permit unambiguous identification of each eye in the resultant MRI image. In the remaining three eyes of the uppermost layer, it is possible to see the ink mark on the nasal cornea, which was used to indicate the original orientation of the eye in the head.