Table 3 of Hou, Mol Vis 2010; 16:1659-1668.
Tissue |
Storage period and storage condition |
Nominal concentration (µg/ml) |
Observed concentration (µg/ml)±SD |
Accuracy (%) |
RSD (%) |
---|---|---|---|---|---|
Plasma | 3 Freeze/thaw cycles(−20 °C) | 1 | 1.06±0.06 | 107.56 | 8.26 |
10 | 10.9±0.24 | 110.25 | 3.81 | ||
100 | 102.12±5.30 | 102.01 | 5.65 | ||
Process sample stability (RT; 8 h) | 1 | 0.98±0.65 | 99.58 | 8.54 | |
10 | 9.86±0.73 | 98.75 | 3.32 | ||
100 | 90.48±0.59 | 90.69 | 7.15 | ||
Long-term stability (−20 °C; One month) | 1 | 0.96±0.08 | 97.18 | 7.92 | |
10 | 9.19±0.84 | 92.51 | 6.39 | ||
100 | 100.82±0.13 | 101.38 | 3.84 | ||
Autosampler stability (4 °C; 8 h) | 1 | 0.91±0.31 | 91.44 | 5.24 | |
10 | 9.31±0.74 | 92.59 | 6.31 | ||
100 | 89.43±0.21 | 89.35 | 4.97 | ||
Eye | 3 Freeze /thaw cycles (−20 °C) | 0.5 | 0.50±0.94 | 108.75 | 9.38 |
2 | 1.98±0.54 | 99.56 | 3.45 | ||
8 | 8.01±0.16 | 102.79 | 2.72 | ||
Process sample stability (RT; 8 h) | 0.5 | 0.46±0.06 | 95.37 | 8.68 | |
2 | 1.97±0.32 | 94.29 | 10.26 | ||
8 | 7.98±0.16 | 98.35 | 7.4 | ||
Long-term stability (−20 °C; One month) | 0.5 | 0.51±0.29 | 105.54 | 6.51 | |
2 | 2.02±0.08 | 101.32 | 8.25 | ||
8 | 7.99±0.11 | 99.16 | 4.03 | ||
Autosampler stability (4 °C; 8 h) | 0.5 | 0.47±0.69 | 91.24 | 2.38 | |
2 | 1.96±0.37 | 96.67 | 4.76 | ||
8 | 7.87±0.46 | 92.54 | 6.43 |