Molecular Vision: Hou, Mol Vis 2010; 16:1659-1668. Table 2

Table 2. Recovery of mangiferin from rat plasma and eye (n=6).

Matrix	Spiked concentration (μg/ml)	Recovery (%±SD)	RSD (%±SD)
Plasma	1	0.825±0.05	6.4
	10	0.832±0.11	13.6
	100	0.931±0.15	14.9
Eye	0.5	0.911±0.24	5.8
	2	0.969±0.06	7
	8	0.923±0.14	14.7

The extraction efficiency of mangiferin was determined by analyzing replicate sets (n=6) of QC samples: 1.00,10.00, and 100.00 μg/ml for rat plasma and 0.50, 2.00, and 8.00 μg/ml for rat eye, representing low, medium, and high QCs, respectively. Recovery was calculated by comparing the peak areas of mangiferin added into blank samples and extracted using the protein precipitation procedure with those obtained from mangiferin spiked directly into post-protein precipitation solvent at three QC concentration levels.