Molecular Vision: Hou, Mol Vis 2010; 16:1659-1668. Table 1

Table 1. Intra- and inter-day precision and accuracy of mangiferin in rat plasma and eye (n=6).

Matrix	Nominal concentration (μg/ml)	Observed concentration (μg/ml)±SD	Accuracy (% bias)	RSD (%)
Plasma Intra-day (n=6)	1	0.96+0.08	−4	8.33
	10	9.89±0.51	−1.1	5.15
	100	99.62±6.80	−0.4	6.82
Inter-day(n=6)	1	0.94±0.08	−6	8.51
	10	10.02±1.01	2	10.31
	100	99.48±0.08	−0.5	0.08
Eye Intra-day(n=6)	0.5	0.49±0.04	−2	8.16
	2	2.02±0.18	2	9.09
	8	7.97±0.19	−0.4	2.38
Inter-day(n=6)	0.5	0.48±0.04	−4	8.33
	2	1.95±0.23	−2.5	11.79
	8	8.01±0.08	0.1	1.01

The intra- and inter-assay accuracies were expressed as the percent difference between the measured concentration and the nominal concentration. Intra-assay precision and accuracy were calculated using replicate (n=6) determinations for each concentration of the spiked plasma sample during a single analytical run. Inter-assay precision and accuracy were calculated using replicate (n=6) determinations of each concentration made on three separate days. Accuracy (% Bias)=[(C_obs -C_nom) /C_nom]×100.The precision (relative standard deviation; RSD) was calculated from the observed concentrations as follows: RSD=[standard deviation (SD) / C_obs] ×100. Accuracy (Bias) and precision (RSD) values within ±15% covering the range of actual experimental concentrations were considered acceptable [2].