Figure 3 of Hou, Mol Vis 2010; 16:1659-1668.


Figure 3. HPLC chromatograms of mangiferin in the eye. HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C18—MS—IIanalytical column (4.6 mm×250 mm, 5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60,v:v). Typical chromatograms of blank eye, blank eye spiked with mangiferin and I.S., and rat eye sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the eye after the protein precipitation step. A: Typical chromatogram of blank eye sample. B: Typical chromatogram of blank eye sample spiked with standard mangiferin (1 μg/ml) and I.S. C: Typical chromatogram of eye sample containing mangiferin (5.63 µg/ml) collected at 1 h after mangiferin administration (50 mg/kg, i.v.).