Figure 4. Representative immunohistochemical analyses of NTG (70 F) and control (72F) donor optic nerve sections. A: Immunoreactivity for protein-bound citrulline detected by a rabbit polyclonal antibody to citrulline after monoxime modification as described in methods. B: Merged image of anti-citrulline with DAPI. C: Control donor section probed for immunoreacitivty with anti-citrulline. D: Merged image of anti-citrulline and DAPI for control donor section. E: Densitometric analysis of immunohistochemical detection of protein-bound citrulline in NTG eyes. The data was analyzed using Image J software.