Table 1 of Xu, Mol Vis 2010; 16:1593-1600.

Table 1. Sequence of primers used to amplify the coding regions of the RS1 gene




Exon


Primers (5′-3′)		 Size of
amplified
fragment
(bp)
Annealing
temperature
(°C)
1 CTCAGCCAAAGACCTAAGAAC 216 61
  GTATGCAATGAATGTCAATGG    
2 GTGATGCTGTTGGATTTCTC 176 59
  CAAAGTGATAGTCCTCTATG    
3 CTGCCCTGCCTCTCTGGTTG 178 59
  GGTGTTCCCAATGACTGTTCC    
4 GGTGCTTGTTGAGTATTGAG 219 62.5
  AAAATCCCCGGGCCCTGC    
5 GAGAGCCAGCACCTGCGG 311 65
  GGGTGCGAGCTGAAGTTGG    
6 CCCGATGTGATGGTGACAGG 414 58
  CTTTGTTCTGACTTTCTCTGGC    

This table summarizes the primer sequences, size of the amplified fragments and the annealing temperatures for each primer pairs.

Xu, Mol Vis 2010; 16:1593-1600. http://www.molvis.org/molvis/v16/a171

©2010 Molecular Vision http://www.molvis.org/molvis/

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